University

Deployment Methods

Injection, moisturiser cream, nasal spray. Three routes, three sets of rules.

Measure twice Wrong units and wrong volumes are the most common screw-ups in peptide research. This page is education, not medical advice.

Table of Contents

Pick Your Route
Subcutaneous Injection
Topical Moisturiser
Nasal Spray
Units and Math
Reference Tables
Common Mistakes
Checklists

Pick Your Route

Most protocols assume subcutaneous injection: reconstitute with BAC, draw with an insulin syringe, inject into fatty tissue under the skin.

Two other routes show up often enough to matter:

Topical moisturiser: reconstituted peptide mixed into plain cream (GHK-Cu is the classic). Applied to skin. Not injected.
Nasal spray: reconstituted in BAC, diluted with sterile saline, loaded into a sprayer.

The Dosage Calculator handles all three. Pick your method and enter your numbers.

Subcutaneous Injection

U-100 insulin syringes are the standard. Thin needles (29G–31G), small volumes, pre-attached tip. Belly, outer thigh, and upper arm are common sites.

Reading U-100

100 units = 1 mL. Each unit is 0.01 mL. Syringe "units" are volume only. They are not International Units (IU) and they are not your peptide dose.

1 mL barrel: up to 100 units. Default for most people.
0.5 mL barrel: up to 50 units. Easier to read small draws.
0.3 mL barrel: up to 30 units. Best for very tiny volumes.

Reading the plunger

Confirm U-100 on the barrel.
Read at the top flat edge of the rubber stopper, eye level.
Debubble before you trust the reading.

Needles

30G × ½ inch is the usual recommendation. 29G if the solution is thick. 31G if you want the thinnest option.

Injection math Concentration = peptide in vial ÷ BAC water. Dose volume = target dose ÷ concentration. Syringe units = dose volume × 100.

Topical Moisturiser

Common with GHK-Cu and similar skin-focused peptides. Reconstitute normally, then blend the liquid into a plain, fragrance-free moisturiser base.

Basic flow

Reconstitute peptide vial with BAC water.
Weigh your moisturiser base in grams.
Mix peptide solution into cream with clean technique.
Refrigerate. Label with date.

How much cream per use

Most people use a pea-sized dab, roughly 0.25–0.5 g. A fingertip unit (FTU) is often ~0.5 g. That's a visual guide, not a syringe measurement.

Peptide per application = concentration in your final mix (mcg per gram) × grams you apply. The calculator's topical mode does this if you enter vial size, BAC volume, moisturiser weight, and target dose.

Do not inject topical mix Cream prepared for skin is not sterile for injection, even if you used BAC water to reconstitute.

Nasal Spray

Used when mucosal absorption is the goal. Typical flow: reconstitute in BAC, add sterile saline to hit target concentration, transfer to a nasal bottle.

Basic flow

Reconstitute in BAC water.
Add sterile saline to reach total volume and concentration you want.
Load a clean nasal sprayer (know its output per pump).
Refrigerate. Label with date.

Liquid per spray

Every sprayer is different. Common range 0.05–0.15 mL per pump. Peptide per spray = concentration (mcg/mL) × mL per spray. If you don't know your sprayer's output, you don't know your dose.

Total volume = BAC water + saline added. Calculator nasal mode takes saline volume and spray output alongside your target dose.

Why add saline? BAC preserves the stock vial. Nasal protocols often dilute further with saline to lower concentration and match the tiny volume each spray delivers.

Units and Math

mg and mcg

Peptide mass. What's on the vial label and in most protocols.

1 mg = 1,000 mcg Mixing these up is a thousand-fold error. Check every time.

mL and syringe units

Liquid volume. Meaningless for peptide content until you know concentration.

IU

Biological potency. Mainly HGH (~3 IU per 1 mg somatropin) and HCG (labelled by IU). Most peptides use mg or mcg.

Use the Dosage Calculator

Reference Tables

mg to mcg

mg	mcg
0.1 mg	100 mcg
0.25 mg	250 mcg
0.5 mg	500 mcg
1 mg	1,000 mcg
2 mg	2,000 mcg
5 mg	5,000 mcg

mL to U-100 units

mL	U-100 units
0.01 mL	1 unit
0.05 mL	5 units
0.10 mL	10 units
0.20 mL	20 units
0.25 mL	25 units
0.50 mL	50 units
1.00 mL	100 units

HGH: IU to syringe units (10 IU vial + 1 mL BAC)

IU	mg (approx.)	U-100 units
1 IU	0.33 mg	10 units
2 IU	0.67 mg	20 units
3 IU	1.00 mg	30 units
5 IU	1.67 mg	50 units
10 IU	3.33 mg	100 units

HGH table assumes 10 IU in 1 mL BAC. Different BAC volumes change the unit draw.

Common Mistakes

Syringe units vs IU"2 IU HGH" is not draw to the 2 mark. Calculate from concentration.

mg vs mcgRead the label. Read the protocol. Read it again.

Injecting cream mixTopical is for skin only.

Guessing spray outputNo mL per pump = no idea how much peptide per spray.

Air bubblesBubble volume is not peptide volume. Your dose is short.

Checklists

Injection

Confirm dose unit (mg, mcg, or IU).
Calculate concentration and volume.
Convert to U-100 units (× 100).
Fresh syringe. Draw, debubble, read plunger top edge.

Topical moisturiser

Reconstitute. Note mg/mL.
Weigh moisturiser (g). Mix clean.
Know mcg per gram in final cream.
Pea-sized dab (~0.25–0.5 g). Refrigerate batch.

Nasal spray

Reconstitute in BAC. Add saline to target volume.
Know sprayer mL per pump.
Calculate mcg per spray.
Compare to target. Adjust dilution if needed. Refrigerate.